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Increased intake of lutein and zeaxanthin may help reduce the likelihood of constipation and thus contribute to enhancing quality of life (Table 1) [104, 106, 109]

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Primary antibodies, diluted 1:100, were incubated in a solution of 0.05% tween-20 and 5% normal goat serum (Jackson ImmunoResearch Laboratories, Pennsylvania, USA) in PBS for 1 h at 37 C